Method for Rapid Detection of Bisultap Residues in Strawberry and Its Products Using Ultra Performance Liquid Chromatography with Electrospray Tandem Mass Spectrometry

A ultra performance liquid chromatography with electrospray tandem mass spectrometry(UPLC-MS-MS) method was developed for the quantitative determination of bisultap residue in raw strawberry and its processed products.The samples were extracted by methanol-water(1:1,V/V) without any purifying treatments,were separated by ACQUITY UPLC HSS T3 UPLC column with equal gradient mobile phase,and detected by electrospray tandem mass spectrometry with ES-and multiple reaction monitoring(MRM) modes.Results showed that the mean recoveries range from 65.3% to 91.5% with relative standard deviations in the range of 7.8%～11.8% when the spiked concentrations of bisultap are 0.05,0.10 and 0.20 mg/kg respectively,and the limit of detection is 3.0 μg/kg.The detection operation can be completed within 1 minute with high sensitivity and accuracy,and is suitable for the fast qualitative and quantitative detection for bisultap residue in strawberry samples.