Studies of the expression of CD40~+、CD40L~+ and CD8~+/CD28~+ on peripheral blood cells in patients with chronic B hepatitis

Objective:To test CD40~+、CD40L~+ and CD8~+/CD28~+、CD8~+/CD28~- on peripheral blood of chronic B hepatitis evaluate patients's celluar immunity and to guide clinical treatment.Methods:CD40~+、CD40L~+ and CD8~+/CD28~+、CD8~+/CD28~- expression on peripheral blood cells in chronic B hepatitis were detected by flow cytometry.Results:CD40~+、CD40L~+ and CD8~+/CD28~+ in chronic B hepatitis and liver cirrhosis patients were lower than in the health.CD8~+/CD28~- in chronic B hepatitis and liver cirrhosis patients were higher than in the health.But there were no remarkable difference between in chronic B hepatitis and in liver cirrhosis.Also there were no remarkable difference in mild、moderate、severve chronic B hepatitis and liver cirrhosis (P0.05).There was a positive corrlation on CD40~+ and CD40L~+ in chronic B hepatitis patients.There was a positive corrlation on CD8~+/CD28~+ and CD40~+、CD40L~+ in chronic B hepatitis patients.There were no remarkable corrlation on CD8~+/CD28~- and CD40~+、CD40L~+ in chronic B hepatitis patients.Conclusion:The costimulation molecules CD40~+、CD40L~+ and CD8~+/CD28~+ are lower,while CD8~+/CD28~- are higher in chronic B hepatitis patients than in the health.To test the expression of CD40~+、CD40L~+ and CD8~+/CD28~+ on peripheral blood of the chronic B hepatitis could help to evaluate patients's celluar immunity and guide clinical treatment.