ScholarLens Back to search
2010•Hubei nongye kexueRequires access

Study on the Efficiency of Transforming pKD46 into Competent Cells from Escherichia coli K12

Duo Zhang

Open publisher page 0 citations

Abstract

A series of Escherichia coli strain K12 competent cells were produced in different conditions, including prepared methods, growth status, hot shocked time, etc.The effects of different factors on the efficiency of transforming plasmid pKD46 were studied.The highest transformation ability was 5.4×106 when the activated cells was cultured to an OD600 0.43, prepared with RbCl and hot shocked at 42℃ for 90 s.Meanwhile, the other factors affected the transformation efficiency were optimized.

About this research paper

What this paper is about

A series of Escherichia coli strain K12 competent cells were produced in different conditions, including prepared methods, growth status, hot shocked time, etc.The effects of different factors on the efficiency of transforming plasmid pKD46 were studied.The highest transformation ability was 5.4×106 when the activated cells was cultured to an OD600 0.43, prepared with RbCl and hot shocked at 42℃ for 90 s.Meanwhile, the other factors affected the transformation efficiency were optimized.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

A series of Escherichia coli strain K12 competent cells were produced in different conditions, including prepared methods, growth status, hot shocked time, etc.The effects of different factors on the efficiency of transforming plasmid pKD46 were studied.The highest transformation ability was 5.4×106 when the activated cells was cultured to an OD600 0.43, prepared with RbCl and hot shocked at 42℃ for 90 s.Meanwhile, the other factors affected the transformation efficiency were optimized.

Key concepts: Escherichia coli, Plasmid, Transformation (genetics), Strain (injury), Transformation efficiency, Microbiology, Chemistry, Biology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Study on the Efficiency of Transforming pKD46 into Competent Cells from Escherichia coli K12 — Research Paper | ScholarLens