Study on rpoB mutation in rifampin resistants mycobacterium tuberculosis

Objective To study the rpoB gene mutation in M.tuberculosis isolates,and evaluate their clinical value. Method 60 clinical isolates of mycobacterium tuberculosis were detected using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Results SSCP pattern of reference mycobacterium tuberculosis H37Rv as control, total mutation rate of 60 clinical isolates of mycobacterium tuberculosis is 53.3%(32/60), 4 stains mutation was round in rifampin-susceoitible 24 strains,mutation rate of 16.7%(4/24). SSCP patterns of rifampin resistant clinical isolates were different from the normal control, mutation rate of 77.8%(28/36) is higher than that of rifampin-susceoitible stains(P0.05). Conclusions The results confirm that rpoB gene mutation is the key mechanism in rifampin resistant tuberculosis mutation. It is feasible that using PCR-SSCP to detect drug resistance in mycobacterium tuberculosis.