Defection rifampin-resistant Mycobacterium tuberculosis

Objective: Detection rPOB geng mutations in refampin-resistant Mycobacterium tuberculosis using Polymerase Chain reaction-single-strand conformation polymorphi RFP(PCR-SSCP) and DNA sequencing. Methods:80 clinical isolates of Mycobacterium tuberculosis (32 drug susceptible,48 rifampin-resistance or multidrug resistance including rifampin) by DNA sequencing and PCR-SSCE.Results: No mutation was found in 32 rif-rensitive strains by DNA sequencing. While 44 of 48 rif-rensitive strains had mutations rifampin resistant determination region of rpoB gene, the mutation rate was 91.7%(44/48),531-Ser and 526-His were the most common positions to be substituted.32 rif-rensitive strains had same PCR-SSCP gelprofile with Mycobacterium tuberculosis H37Rv as control, while 45 of 48 rif-rensitive strains showed different SSCP. The specificity is 93.1%. Conclusion:DNA sequencing is significant in drug choosing for tuberculosis treatment. Rifampin-resistant of Mycobacterium tuberculosis is rapidly and accurately detected using PCR-SSCP.