ISSR analysis on genetic relationships of Erigeron breviscapus germplasm resources.

Objective To investigate the genetic relationships of Erigeron breviscapus at the molecular biology level. Methods Thirty six germplasm resources of E.breviscapus collected from Yunnan,Sichuan,and Guizhou Provinces in 2005 were analyzed by inter-simple sequence repeat(ISSR) and cluster analysis based on NTSYS2.Results A total of 13 primers were screened and 125 bands were amplified,among which 103 bands(82.40%) were found to be polymorphic.Thirty six germplasm resources of E.breviscapus were clustered into five groups at genetic distance 0.54,the group Ⅰ included five germplasm resources collected from Chuxiong,Zhaotong,and Diqing of Yunnan Province; the group Ⅱ included 25 germplasm resources collected from Guizhou,Sichuan,and Northwest of Yunnan Province;group Ⅲ included one germplasm resource collected from Wenshan of Yunnan Province;the group Ⅳ included one germplasm resource collected from Qujing of Yunnan Province;the group Ⅴ included two germplasm resources collected from Diqing of Yunnan Province.Conclusion There is abundant genetic diversity in the germplasm resources of E.breviscapus,and the genetic relationships are closely related to geographical distance where they are collected.