Application of brilliant cresyl violet in demonstrating Nissl bodies of neuron in sciatic nerve injury
Xiao Yan
Abstract
Xiao Yan
Abstract
Objective To explore the way of demonstrating neurons in sciatic nerve injury.Methods A model for resection of sciatic nerve was used. L4-6 spinal cord was taken,and sectioned (paraffin embectling) and stained in brilliant cresyl violet.Results The number for magnocellular and middle cellur neurons in lateral nucleus of L4 6 spinal anterior horn of injury animals was lower than that of normal control, with a blurred profile of Nissl bodies.Conclusion The visualization of Nissl body with staining brilliant cresyl violet is available in the morphological study of nerve injury,including sciatic nerve injury.
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Objective To explore the way of demonstrating neurons in sciatic nerve injury.Methods A model for resection of sciatic nerve was used. L4-6 spinal cord was taken,and sectioned (paraffin embectling) and stained in brilliant cresyl violet.Results The number for magnocellular and middle cellur neurons in lateral nucleus of L4 6 spinal anterior horn of injury animals was lower than that of normal control, with a blurred profile of Nissl bodies.Conclusion The visualization of Nissl body with staining brilliant cresyl violet is available in the morphological study of nerve injury,including sciatic nerve injury.
Key concepts: Cresyl violet, Nissl body, Medicine, Sciatic nerve, Anatomy, Staining, Sciatic nerve injury, Spinal cord