Proliferation Study of Hippocampal Neural Stem Cells in Dentate Gyrus of Depressed Model Rats

Objective To evaluate the hippocampal neurogenesis in depression.Methods A total of 20 Wistar rats were randomly divided into control and depression group(model group).Chronic unpredicted stress model was established.Body mass weigh and behavioral scores of the rats were observed.Radioimmunoassay was used to measure plasma concentrations of corticotropin releasing hormone,adrenocorticotropic hormone and cortisone,Light and electron microscope were used to detect the morphological change of hippocampal CA3 neurons.Immunohistochemistry and in situ hybridization were used to detect both protein and mRNA expressions of Nestin in hippocampal dentate gyrus.Results Compared with the control group,rats in the model group,both body mass weigh and the related increase rate were markedly decreased,and the behavioral scores were reduced significantly.In addition,neural damage was observed in hippocampal CA3 neurons,and the Nestin expressions in hippocampal dentate gyrus were evidently increased.Conclusion Hippocampal neurogenesis was increased in depression.