Extraction technology for total fiavonoids from Polygonum aviculare by orthogonal design

Objective To use the Soxhlet extractor for the extract of total flavonoids from Polygonum aviculare and determine the flavonoid content in Polygonum aviculare by UV spectrophotometry.Methods The best extraction technology was obtained by the way of orthogonal experiment according to the content of total flavonoids.Results The optimum conditions were as follows:the extraction solution concentration was 1∶ 40(1 g of Polygonum aviculare:40 L of 70% ethanol),extraction solvent was 70% ethanol,extraction temperature was 85 ℃,extraction time was 1.5 h.Conclusions The best technology for the extraction of total flavonoids is obtained with extract ratio of 15.40%,the content of total flavonoids is 0.308%,the recovery is between 99.8% and 102.4%,RSD is 0.957%(n=5).