ScholarLens Back to search
2011•Xumu shouyi xuebaoRequires access

Cadmium Induced Apoptosis in Rat Hepatocytes by Calcium Overloading

Liu Zong-ping

Open publisher page 2 citations

Abstract

The aims of this study were to explore the effect of i on the apoptosis of rat hepatocytes induced by cadmium.Rat hepatocytes were isolated by a two-step perfusion technique.After 24 h planting,hepatocytes were treated with cadmium in the presence or absence of Bapta-AM.Cell viability was evaluated with a colorimetric using MTT level.Cell morphology and apoptosis were photographed under inverted and fluorescence microscope.i,ROS generation and ΔΨm collapse were evaluated with flow cytometry.The results showed sharp deformation,necrosis and apoptosis in hepatocytes with the dose of Cd increased and time prolonged.Cd induced i increased,ΔΨm collapse and ROS generation(P0.01).Bapta-AM effectively inhibited Cd-induced cytotoxicity(P0.05 or P0.01).It was showed that cadmium induced apoptosis in rat hepatocytes by calcium overloading.

About this research paper

What this paper is about

The aims of this study were to explore the effect of i on the apoptosis of rat hepatocytes induced by cadmium.Rat hepatocytes were isolated by a two-step perfusion technique.After 24 h planting,hepatocytes were treated with cadmium in the presence or absence of Bapta-AM.Cell viability was evaluated with a colorimetric using MTT level.Cell morphology and apoptosis were photographed under inverted and fluorescence microscope.i,ROS generation and ΔΨm collapse were evaluated with flow cytometry.The results showed sharp deformation,necrosis and apoptosis in hepatocytes with the dose of Cd increased and time prolonged.Cd induced i increased,ΔΨm collapse and ROS generation(P0.01).Bapta-AM effectively inhibited Cd-induced cytotoxicity(P0.05 or P0.01).It was showed that cadmium induced apoptosis in rat hepatocytes by calcium overloading.

Why it matters

OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The aims of this study were to explore the effect of i on the apoptosis of rat hepatocytes induced by cadmium.Rat hepatocytes were isolated by a two-step perfusion technique.After 24 h planting,hepatocytes were treated with cadmium in the presence or absence of Bapta-AM.Cell viability was evaluated with a colorimetric using MTT level.Cell morphology and apoptosis were photographed under inverted and fluorescence microscope.i,ROS generation and ΔΨm collapse were evaluated with flow cytometry.The results showed sharp deformation,necrosis and apoptosis in hepatocytes with the dose of Cd increased and time prolonged.Cd induced i increased,ΔΨm collapse and ROS generation(P0.01).Bapta-AM effectively inhibited Cd-induced cytotoxicity(P0.05 or P0.01).It was showed that cadmium induced apoptosis in rat hepatocytes by calcium overloading.

Key concepts: Apoptosis, Cadmium, Biology, Flow cytometry, Calcium, Cytotoxicity, Fluorescence microscope, Necrosis

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Cadmium Induced Apoptosis in Rat Hepatocytes by Calcium Overloading — Research Paper | ScholarLens