Cluster and principal coordinate analysis among 12 beer barley by SSRs

The genetic relationships among 12 beer barley varieties with known origin were analyzed by SSRs to provide credible genetic information in breeding beer barly.160 distinctive loci in total were amplified from 25 pairs of SSR primers,and 136 were polymorphic,which accounted for 85.0%.1 to 13 polymorphic loci could be amplified from each primer,with an average of 5.44.The 12 beer barley varieties could be classified into 3 groups and 2 subgroups at the level of SM0.63 by cluster analysis,while 2 groups and 4 subgroups could be sorted by principal coordinate analysis.The two methods above exhibited similar phylogenesis among the tested cultivars.However,more authentic genetic relationship among the 12 beer barley varieties could be provided by the principal coordinate analysis.