2005•Yaowu fenxi zazhiRequires access

HPLC Determination of Ursolic Acid in Shanzhajing Jiangzhi Tablets

Zhai Hong-yu

Abstract

Objective:To establish a method for determination of ursolic acid in Shanzhajing Jiangzhi tablets.Methods:To determine ursolic acid in Shanzhajing Jiangzhi tablets by HPLC.The liquid chromatography was carried out using a Zorbax 80A Extend -C_(18) column(4.6 mm×250mm,5μm)with detection wavelength at 220 run.The mobile phase:methanol-water-acetic acid-triethylamine (83:17:0.04:0.01).The flow rate was 1.0 mL·min~(-1).Results:The calibration curve was linear in the range of 1.092-16.38μg for ursolic acid (r=1.000).The average recovery(n=5)was 99.2%.Conclusion:The method is rapid,accurate and reliable.

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Key concepts: Chemistry, Ursolic acid, Chromatography, High-performance liquid chromatography, Calibration curve, Triethylamine, Acetic acid, Detection limit