The histochemical study on transpositional nerve for respiratory function rehabilitation in rats model with upper cervical spinal cord injuries

Objective To assess the possibility of anastomosed nerve for respiratory rehabilitation in rats model with upper cervical spinal cord injuries(ucSCI).Methods Sixty male adult rats underwent transposition of accessory nerve and phrenic nerve.Accessory nerve was cut subclavicularly upside the embranchment of the interior and exterior branches,and was sutured with phrenic nerve trunk on both sides.The nerve fibers in 5mm distal an proximal to the anastomosis were get monthly after operation,in ten rats each time.AchE histochemically stain was performed with Kamorsky-roos methods.The motor fibers were analyzed by the VIDS-Ⅲ graphical machine.The passing rate of the motor fibers in the distal anastomosis was calculated.Results The passing rate of motor fibers was 43.06±5.36% at 1 mon,and 97.55±6.72% at 6 mons.Conclusion The motor fibers could pass anastomosis after transposition of accessory nerve and phrenic nerve in rats model with ucSCI.It shows the potential possibility to rehabilitate respiratory function by nerve transporsition.