Effects of RPM on renal function and ultrastructure after ischemia-reperfusion in rats

Objective:To investigate the effect of rapamycin on renal function and ultrastructure changes after renal ischemia-reperfusion in rats.Methods:Acute ischemic renal injury model was established(right kidney ectomized,45 min of left renal ischemia and reperfusion for 24h).Thirty male Wistar rats were randomly divided into 3 groups:sham operation group(control group or group C,without renal ischemia),renal ischemia-reperfusion group(ischemia-reperfusion group or group I,without rapamycin),renal ischemia-reperfusion and(RPM 4mg/(kg·d)×3d,reperfusion at 2h before the last operation) intravenous injection group(rapamycin group or group U).changes of renal function and renal uhrastructure were measured BUN level,serum creatinine values.Results:Serum creatinine(168±37)μmol/L and BUN concentration(22±6)mmol/L in group I were significantly higher than those in group U:serum creatinine(113±17)μmol/L and BUN concentration(13.8±2.3)mmol/L(P0.05).under the observation of electromicrosope,the renal ultrastructure of group Cwas normal,the ultrastructure was greatly injured in group I,large quantity of apoptosis residues were stopped in the renule intracellular lipid accumulation and cell apoptotis presented meanwhile,it was obviously ameliorated in group U.Conclusion:Rapamycin greatly improves renal function and provides remarkable protection on renal ultrastructure after ischemia-reperfusion of kidney in rats.