Cloning and sequencing of fusion glycoprotein gene of goose's paramyxovirus HZ strain

The genomic RNA from purified GPV-HZ strain was extracted and used as a template for PCR.The fragment of the fusion gene was amplified by RT-PCR with the primers designed according to the sequence of NDV from Genbank and cloned into pUCm-T and sequenced.As a result,the ORF of fusion gene was 1662 bp in length,encoding 553 amino acid.By sequence comparison among HZ and ZJ1,F48E9 and Lasota strains,homologies of nucleotide sequence were 98.3%, 85.4% and 82.7% respectively.Homologies of the amino acid were 97.8%,90.6% and 82.7% respectively.The results indicated that the mutation on the F gene had varied largely,compared with classic NDV.