Study on the technology for hydrolysis of casein for digestion and absorption of peptides

In order to improve digestion and absorption of casein,casein hydrolysate was prepared by trypsin,it is easy to digest and absorb.The conditions of hydrolized casein by the trypsin were studied and found that optimize the parameters were as follows:enzyme to substrate ratio(E/S) 14 000 U/100 mL,temperature 50 ℃,pH in the beginning 8,hydrolysis time 2.5 h,mass fraction of substrate 8%.Under the optimum conditions of the trypsin hydroyzate of casein,degree of hydrolysis was 7.6%,mass fraction of molecular weight 2 000~180 was 80%,mass fraction of molecular weight 1 000~180 was 59%.