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Effect of Epigallocatechin-3-gallate(EGCG) on MMP-9 Expression in Human Colon Cancer Cell Line HT-29 in Vitro

YU Xianmin

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Abstract

Objective:To study the effect of Epigallocatechin-3-gallate(EGCG) on Matrix metalloproteinase 9(MMP-9) in human colon cancer cell line HT-29 in vitro.Methods:In vitro,the MMP-9 mRNA and protein expression in human colon cancer cell line HT-29 were analyzed by RT-PCR and Western blot.Results:In vitro,human colon cancer cell line HT-29 was treated by various concentrations of EGCG(25,50,100 mg/L).After 48 hours,the expression of MMP-9 mRNA and protein in human colon cancer cell line HT-29 were gradually decreased with the increasing dose of EGCG(P 0.05).Conclusion:EGCG can inhibit the expression of MMP-9 in HT-29 cell in vitro and in dose dependent manner,which may be one of the mechanisms of anti-tumor effect of EGCG.

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What this paper is about

Objective:To study the effect of Epigallocatechin-3-gallate(EGCG) on Matrix metalloproteinase 9(MMP-9) in human colon cancer cell line HT-29 in vitro.Methods:In vitro,the MMP-9 mRNA and protein expression in human colon cancer cell line HT-29 were analyzed by RT-PCR and Western blot.Results:In vitro,human colon cancer cell line HT-29 was treated by various concentrations of EGCG(25,50,100 mg/L).After 48 hours,the expression of MMP-9 mRNA and protein in human colon cancer cell line HT-29 were gradually decreased with the increasing dose of EGCG(P 0.05).Conclusion:EGCG can inhibit the expression of MMP-9 in HT-29 cell in vitro and in dose dependent manner,which may be one of the mechanisms of anti-tumor effect of EGCG.

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Available abstract

Objective:To study the effect of Epigallocatechin-3-gallate(EGCG) on Matrix metalloproteinase 9(MMP-9) in human colon cancer cell line HT-29 in vitro.Methods:In vitro,the MMP-9 mRNA and protein expression in human colon cancer cell line HT-29 were analyzed by RT-PCR and Western blot.Results:In vitro,human colon cancer cell line HT-29 was treated by various concentrations of EGCG(25,50,100 mg/L).After 48 hours,the expression of MMP-9 mRNA and protein in human colon cancer cell line HT-29 were gradually decreased with the increasing dose of EGCG(P 0.05).Conclusion:EGCG can inhibit the expression of MMP-9 in HT-29 cell in vitro and in dose dependent manner,which may be one of the mechanisms of anti-tumor effect of EGCG.

Key concepts: In vitro, Cell culture, Colorectal cancer, Molecular biology, Epigallocatechin gallate, Western blot, Matrix metalloproteinase, Cancer

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Effect of Epigallocatechin-3-gallate(EGCG) on MMP-9 Expression in Human Colon Cancer Cell Line HT-29 in Vitro — Research Paper | ScholarLens