2009•Journal of Chinese MicrocirculationRequires access

Effect of Epigallocatechin-3-gallate(EGCG) on MMP-9 Expression in Human Colon Cancer Cell Iine HT-29 In Vitro

YU Xian-min, Weichang Chen

Abstract

Objective To study the effect of Epigallocatechin-3-gallate(EGCG) on matrix metalloproteinase 9(MMP-9) in human colon cancer cell line HT-29 in vitro. MethodsIn vitro, the MMP-9 mRNA and protein expression in human colon cancer cell line HT-29 was analyzed by RT-PCR and Western blot. ResultsIn vitro, human colon cancer cell line HT-29 was treated by EGCG in various concentrations(25,50,100μg/ml). After 48 hours,the expression of MMP-9 mRNA and protein in human colon cancer cell line HT-29 were gradually decreased with the increasing dose of EGCG(P0.05).ConclusionEGCG could inhibit the expression of MMP-9 in HT-29 cell in vitro and in dose dependent manner,and maybe it is one of the mechanizms of the anti-tumor effect of EGCG.

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Key concepts: In vitro, Cell culture, Colorectal cancer, Molecular biology, Western blot, Cell, Cancer, Matrix metalloproteinase