2003•Europe PMC (PubMed Central)Requires access
Expression of hepatitis B virus preS 2 protein in transgenic mice liver
Jin Yan-hua, Xiaoyuan Zi, Yao Yucheng, Xiong Jun, Li Jianxiu, Xiaoping Su, Xinmin Wang, Wenjun Ni, Cong Wenming, Yang Kangjuan, Yiping Hu
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Abstract
Following microinjection of codign sequence of pcDNA3.1-preS/S~(t), the embyros were transferred to oviducts of pseudopregnant females. Fifteen pups were born and 7 of them survived, two of them were verified to integrate the preS/S~(t) gene in their genomic DNA by PCR assay, named C57-TgN(preS/S~(t)) SMMU. With immunohistochemistry, preS 2 protein was detected in hepatocytes cytoplasm of C57-TgN(preS/S~(t)) SMMU transgenic mice. Lymphocyte effusion was found near the central vein of liver tissue and NP of hepatocytes increased in transgenic mice by H-E staining. Then the transgenic mice were mated with normal mice to establish transgenic mice strains. Now one of them had F_(2) offsprings.