2000•Zhongguo shouyi xuebaoRequires access
Construction of transgenic mice of human leukocyte antigen B27 (HLA-B27)
Tielin Zhou, Guilin Qiao, Shaofeng Yang, Qifu Wu, Yi Liu, Yuxun Zhang, Yin Zhen
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Abstract
The transgenic mice of human leukocyte antigen B27 (HLA-B27) has been generated by microinjection of the mixture of 6.5 kb HLA-B27 gene and 15 kb beta_(2)-microglobulin (beta_(2)-m) gene into the fertilized eggs of mice. 1 050 out of 1 370 microinjected eggs have been transplanted to 58 pseudopregnant mice and 97 offspring were produced. Genomic DNA samples from the offspring have been detected by PCR and southern blotting techniques. The results showed that 27 out of 97 mice were positive by analysis of PCR and 8 mice were positive by southern blotting detection.