Study on hydrolysis of the high-temperature soybean meal by protamex protease

The hydrolysis characteristics of the high-temperature soybean meal hydrolyzed with protamex protease was studied systematically,by using single factor experiment and orthogonal experiment method. Through the effects of enzyme quantity,temperature,time,pH and substract concentration during the hydrolysis were studied,the optimized conditions of hydrolysis were gotten. Results indicated that the optimum hydrolysis conditions of the high-temperature soybean meal by protamex protease are the following:enzyme quantity 18000U/g,temperature 55℃,time 4h,pH 7.0 and substract concentration 9%.In such a condition,the degree of hydrolysis of the high-temperature soybean could reach 36.8%.