Study on the Detection of Staphylococcus aureus Enterotoxin A Gene in Water by PCR

[Objective] The aim was to establish a rapid specific assay for the detection of Staphylococcus aureu enterotoxin A in drinking water.[Method] A polymerase chain reaction(PCR)assay targeting gene encoding Staphylococcus aureus enterotoxin A was developed for detecting Staphylococcus aureus in pure DNA and artificially contaminated water.Salmonella,E.coli DNA and blank were also amplified under the same condition.Staphylococcus aureus enterotoxin A was amplified by PCR.[Result] The detection result of Staphylococcus aureus DNA was positive,with detection limit being 100 cfu/L;the while PCR products was not found in Salmonella,E.coli and blank.The total assay time including enrichment was approximately 24 h.[Conclusion] Results suggested that the PCR could be used to rapidly detect Staphylococcus aureus in water and possibly other types of food products,and the PCR assay also showed high specificity,sensitivity and easy operation.