Effects of Nano-hydroxyapatite/collagen on Differentiation of Bone Marrow Stromal Cells into Osteoblasts Induced by Platelet-rich Plasma in Vitro

Objective:To explore nano-hydroxyapatite/collagen(nHAC) and platelet-rich plasma(PRP) on inducing rabbit bone marrow stromal cells(rBMSCs) into osteoblasts.Methods:rBMSCs co-cultured with or without nHAC were induced by PRP.The growth state of rBMSCs co-cultured with nHAC was observed by scaning electron microscope.To compare with the ability of PRP inducing rBMSCs co-cultured with or without nHAC into osteoblasts,alkaline phosphatase(ALP) activity,osteocalcin(OCN) content and the mRNA level of osteopontin(OPN) were measured.Results:The osteoblasts differentiated from rBMSCs grew well on nHAC.Compared with the cells cultured conventionally,ALP activity and OCN content of cells co-cultured with nHAC increased greatly.The relative expression level of OPN mRNA under co-culture condition was 4.78 times greater than that of under conventional culture condition.Conclusion:nHAC as scaffold material could improve the ability of PRP to induce the rBMSCs into osteoblasts.