Effect of Parthenolide on Proliferation and Apoptosis of Cultured Human Umbilical Vein Endothelial Cells.

Objective To investigate the effect of parthenolide on proliferation and apoptosis of cultured human umbilical vein endothelial cells(HUVECs).Methods HUVECs were exposed to parthenolide in different concentration for different time length.MTT was used to detect the proliferative vitality of HUVECs.The apoptotic cells were determined by TUNEL.Results Compared with that of the control group,parthenolide at the concentration of 1 or 5μmol/L has no effect on the vitality of HUVECs(P0.05),whereas 10～20μmol/L parthenolide can inhibit proliferation of HUVECs(allP0.01) with dose-and time-dependent manner.The apoptotic index of HUVECs was not affected after exposure to parthenolide at the concentration of 5μmol/L.Parthenolide at the concentration of 10 and 15μmol/L both have higher apoptotic index than that of control group at 6h,12h and 24h.(P0.01),and that was more significant with the parthenolide concentration of 10 and 15μmol/L at 12h and 24h(P0.01).Conclusions Parthenolide in low concentration has no effect on proliferation and apoptosis of HUVECs,while higher concentration of parthenolide can inhibit proliferation and induce apoptosis of HUVECs.