Optimization of culture conditions for liquid spawn of Pleurotus eryngii in factory cultivation

The effects of medium initial pH value,nitrogen source,carbon source,culture temperature,inoculum,ventilation,shaking speed on liquid spawn cultivation of Pleurotus eryngii were discussed to determine the optimum culture conditions through the single factor screening method.The results showed that the optimum initial pH was 6,the optimum nitrogen source were yeast powder and bean cake powder,the suitable carbon source was glucose,the optimum culture temperature was 27 ℃,the optimum inoculum was 12%,the optimum shaking speed was 123 r/min,and the better ventilation mode was gauze packing.