High frequency shoot regeneration from Giant Duckpear leaves in vitro

Tube seedlings of Giant Duckpear which was a sport of Duckpear( Pyrus bretschneideri Rehd ) were selected as materials, with young expanded leaves extracted as explants Effect of element composition of basic media, foreign hormone and leaf orientation inoculated on media on shoot regeneration was studied The results showed that the element composition of basic media was the major factor affecting shoot regeneration Foreign hormones directly affected shoot regeneration frequency Leaf orientation inoculated on media affected average shoot number per leaf The optimal medium of inducing high frequency shoot regeneration was NN69 supplemented with TDZ and IBA Average shoot number per leaf was hightest when the leaf abaxial side touched media