Study on shoot regeneration from leaf explants in peach

【Objective】 Factors affecting shoot regeneration from leaf explants in vitro were investigated in peach in order to establish and optimize shoot regeneration system.【Method】 Experiment was conducted with Bumuzaosheng,Tiantaowang,Ruijiang and 97-8-4 as test materials to study the effects of different genotypes,and different leaf ages,different basic media,different growth regulators on shoot regeneration of leaf explants from tissue culture plantlets in peach.【Result】 Shoot regenerating rate differed greatly from leaf explants in different genotypes.Leaf explants of 97-8-4 and Tiantaowang showed high shoot regeneration rate.Leaf explants of Bumuzaosheng were less efficient.Leaf explants of Ruijiang was unable to regenerate.Shoot regnerating rate was different in different basic media and LP was the optimum basic medium for shoot regeneration.Shoot regenerating rate differed from leaf explants in different maturity and the fully expanding young leaf was the optimum explant for shoot regeneration.Shoot regenerating rate was different from different parts of leaf explants.The petiole and the base of leaf were efficient for shoot regeneration.In dark culture the optimum growth regulators were 2.0 mg/L BA with 0.2 mg/L NAA for shoot regeneration.In dark-light culture,the optimum growth regulators were 4.0-6.0 mg/L BA and the concentration of BA/NAA was 30∶1-60∶1.Shoot regenerating rate from fully expanding young leaf explants of 97-8-4,Tiantaowang and Bumuzaosheng was 18.33%,16.67% and 13.33% under optimum culture condition,respectively.【Conclusion】Shoot regeneration was afftected by internal and external factors from leaf explants in peach.The genotype and the physiological state of leaf explants were the crucial internal factors for shoot regeneration.The basic medium and the growth regulators were the crucial external factors for shoot regeneration.