Study on optimization of the extraction technology of total flavonoids from strawberry by orthogonal test

The extraction technology of total flavonoids from strawberry was optimized in this paper.Based on single factor test,the effect of ethanol concentration,extraction time,extraction temperature,solid/liquid ratio(g/mL) on the extraction rate of total flavonoids was studied according to L9(34)orthogonal test.The results showed that factors influencing the extraction of total flavonoids from the strawberry by the water bath method were in the order as follows: ethanol concentrationsolid/liquid ratio(g/mL)extraction timeextraction temperature,the optimum extracting conditions of total flavonoids from the strawberry for orthogonal test were : ratio of solid/ liquid was 1∶ 35,alcohol concentration 55%,extracting time 130 minutes and extracting temperature was 60°C.The extraction rate of total flavonoids was 5.13 mg/g under the above optimal extracting conditions.