Study on the FSHβ gene of Maiwa yak and Jiulong yak by PCR-SSCP
Yang Qi-yuan
Abstract
Yang Qi-yuan
Abstract
The FSHβ,s,5,flank region of the Maiwa yak and Jiulong yaks is amplified by using a pair of special primers and its polymorphism is analyzed by PCR-SSCP.The results show that three genotypes AA,BB,AB can be dectected in the FSHβ,s,5,flank region of Maiwa yak,but only AA and AB in Jiulong yaks.Frequency of AA genotype is the highest,and that of A allele is obviously higher than that of B allele in two yak breeds.The PIC was 0.3431 and 0.1411 in Maiwa and Jiulong yak respectively.The Maiwa yaks loci have higher polymorphism and larger variation.
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The FSHβ,s,5,flank region of the Maiwa yak and Jiulong yaks is amplified by using a pair of special primers and its polymorphism is analyzed by PCR-SSCP.The results show that three genotypes AA,BB,AB can be dectected in the FSHβ,s,5,flank region of Maiwa yak,but only AA and AB in Jiulong yaks.Frequency of AA genotype is the highest,and that of A allele is obviously higher than that of B allele in two yak breeds.The PIC was 0.3431 and 0.1411 in Maiwa and Jiulong yak respectively.The Maiwa yaks loci have higher polymorphism and larger variation.
Key concepts: YAK, Biology, Polymorphism (computer science), Genotype, Genetics, Single-strand conformation polymorphism, Allele, Gene