Study on the FSHβ Gene of Maiwa Yak and Jiulong Yak by PCR-SSCP

The FSHβ′s′5′ flank region of the Maiwa yak and Jiulong yaks was amplified by using a pair of special primers and its polymorphism was analyzed by PCR-SSCP.The results showed that three genotype AA,BB,AB were dectected in the FSHβ′s′5′ flank region of Maiwa yak,but only AA and AB in Jiulong yaks.Frequency of AA genotype was the highest,and frequency of A allele was obviously higher than that B allele in two yak breeds.The PIC were 0.3431 and 0.1411 in Maiwa and Jiulong yak respectively.The Maiwa yaks loci have higher polymorphism and larger variation.