ESTABLISHMENT OF MODIFIED RAT MODEL WITH CHRONIC ATROPHIC GASTRITIS INFECTED BY HELICOBACTER PYLORI

Objective To create a more ideal rat model of chronic atrophic gastritis(CAG) infected by Helicobacter pylori(Hp).Methods Sixty male Wistar rats were divided into four groups in random-group D(model group,Hp,sodium salicylate and ethanol group),group A(normal control group),group B(merely-Hp infected group),and group C(sodium salicylate and ethanol group).The Hp-infected CAG models were created by intragastric vaccination of Hp strain(SSl) and intragastric administration of mixed solution of sodium salicylate and ethanol for 14 weeks.The field planting in each group was detected and the histopathological markers of mucosa of gastric antrum were scored.Results Colonization of Hp in gastric mucosa was found in both model group and group B.The antral gastric mucosa in model group became thinner,the numbers of glands in lamina propria moderately decreased,accompanying with moderately chronic active inflammation.The score of chronic inflammation,active inflammation and decrease of intrinsic gland in model group was significantly higher than that in groups A and B(χ2=47.632-48.276;q=6.514-11.191;P0.01),compared with group C,the difference was not significant(P0.05).Conclusion The causes of disease and pathological changes in rat models of CAG that created by Hp infection combined with mixed solution of sodium salicylate and ethanol are similar to that found in human subjects.This can be used as a suitable animal model in researching the mechanism of CAG due to Hp infection and drug therapy for this condition.