Study of real time TaqMan PCR for detction of Enterobacter sakazakii

Objective To establish the rapid detection assay of Enterobacter sakazakii and provide reliable technical basis for clinical application.Methods In order to evaluate the sensitivity,specificity and stability of the real time TaqMan PCR assay to detect Enterobacter sakazakii in feces specimens,we made the feces simulation specimens with the concentrations of Enterobacter sakazakii from 1.2×100 to 1.2×108 cfu/ml by 10 series dilutions of Enterobacter sakazakii strain and mixing well with healthy human feces.Results The sensitivity of real time TaqMan PCR assay of the feces simulation specimens was 1.2×103 cfu/ml,and the range of the linearity was from 1.2×103 to 1.2×108 cfu/ml,the stability evaluation indicated that the coefficient of variation was from 0.65% to 1.47%,and the specificity was 100%.Conclusion The real time TaqMan PCR assay developed in our study could be used in the detection of Enterobacter sakazakii in feces specimens.