The effect of rhbFGF and rhBMP-2 on the proliferation of rabbit osteoblast-like cells

Objective:To study the effect of recombinant human basic fibroblast growth factor(rhbFGF) and recombinant human bone morphogenetic protein-2(rhBMP-2 )with different concentrations on the proliferation of osteoblast-like cells obtained from rabbit bone marrow stromal cells by induced mineralization.Method:The osteoblast-like cells were obtained from rabbit bone marrow stromal cells by induced mineralization. The osteoblast-like cells subcultured to the 2nd passage were used for these experiments. The cells were seeded separately into 96 well plates with approximately 2×10 5 cells/well and incubated in different mediums. The samples were devided into 8 groups by different conditional mediums, included ① Group A:1 ng/ml rhbFGF, ② Group B:10 ng/ml rhbFGF, ③ Group C: 50 ng/ml rhbFGF, ④ Group D: 100 ng/ml rhBMP-2, ⑤ Group E: 1 ng rhbFGF+100ng rhBMP-2/ml, ⑥ Group F: 10 ng rhbFGF+100 ng rhBMP-2/ml, ⑦ Group G: 50 ng rhbFGF+100 ng rhBMP-2/ml, ⑧ Group H: the blank group. Then, the proliferation of osteoblast-like cells were measured after 1day, 4 days, 7days, 10days by MTT methods.Result:The effect was different of the groups of combined growth factors.The effect was the strongest for the proliferation of rabbit osteoblast-like cells with 10ng rhbFGF and 100 ng rhBMP-2/ml.With lower concentration of rhbFGF(1 ng/ml),it could improve the activity of ALP while had nothing to do with the proliferation. With higher concentration of rhbFGF(50 ng/ml).Conclusion: rhbFGF and rhBMP-2 may have synergistic effect on bone formation.The function may be modified by the concentrations of both growth factors. The effect was the strongest for the proliferation of rabbit osteoblast-like cells with 10 ng rhbFGF and 100 ng rhBMP-2/ml.