Daunorubicin induce apoptosis and inhibit proliferation in Jurkat cell lines and its molecular mechanisms

Objective To study the effect of daunorubicin on Jurkat cell line apoptosis induction in vitro and its associated mechanisms.Methods The apoptosis of Jurkat cell line was detected by Annexin V/PI in flow cytometry(FCM) bcl-2,PCNA protein expression was observed by immunocytochemistry.Results Treated with 0.1-2.0μmol/L DNR,the percentage of apoptotic Jurkat cells increased following the drug dose as well as the time extending.Whereas,the percentage of apoptotic Jurkat cells was decreased and cells died absolutely when the drug dose was over 2.0μmol/L or cells were treated 48h with 2.0μmol/L DNR.It was showed that bcl-2,PCNA protein expression level of Jurkat cells treated with 0.5μmol/L DNR for 24h were lower than that of without drug.Conclusion Low dose DNR can efficiently induce apoptosis of Jurkat cell line in vitro.bcl-2 and PCNA gene participate the progress of DNR inducing apoptosis of Jurkat cell line.