Diversity analysis of the endophytic fungi in root and fungi in rhizosphere soil of tea plant in different seasons

A total of 2 019 fungi were isolated from root endophyte and rhizosphere soil of tea plant(Camellia sinensis(L.)O.Kuntze)in different seasons,including 826 endophytic fungi and 1 193 fungi in the rhizosphere soil.The amplification and analysis of rDNA-ITS sequence of the fungi revealed that a total of 28 species of 13 genera of fungi were identified,which includes 12 species of endophytic fungi and 20 species of fungi in the rhizosphere soil.The diversity analysis result of the fungi isolated from different seasons indicated that the difference of Shannon-Wiener diversity index was insignificant among endophytic fungi from three seasons,including 12 species from spring,11 species from summer and 12 species from autumn,while evenness index from summer was the highest,followed by autumn and spring.Species of fungi in the rhizosphere soil from spring,summer and autumn were 18,17 and 20,respectively.Shannon-Wiener diversity index and evenness index of fungi in the rhizosphere soil had no significant difference.Compared to the root endophytic fungi,Shannon-Wiener diversity index and evenness index of fungi in the rhizosphere soil in spring and autumn had significant difference and were higher,but both had no significant difference in summer.