Study on quality standard of YuQuan Pills

OBJECTIVE To establish a qualitative method for identification of Puerariae Radix and Trichosanthis Radix and develop a method for determination of puerarin in YuQuan Pills.METHODS TLC was employed to identify Puerariae Radix and Trichosanthis Radix;HPLC was used to determine puerarin,with A Shimadzu C18 column(4.6mm×250mm,5μm)was used with a mobile phase of methanol-0.1%phosphoric acid solution(75∶25),at a flow rate of 1.0mL·min-1.The detecting wavelength was set at 254nm.RESULTS Puerariae Radix and Trichosanthis Radix can be detected by TLC;Puerarin calibration curve was linear whthin the range of 0.01803～0.9016μg(r=0.99993),and the average recovery of puerarin was 99.61%(RSD=0.67).CONCLUSION The TLC method can be used for qualitative analysis of kudzu root and Radix Trichosanthis,The HPLC method can be used for the content determination of Puerarin.The TLC and HPLC method shows a good separation,reproducity and accuracy.It can be used for quantitative analysis of Radix Puerariae in YuQuan Pills.