Acquisition and Identification of Transgenic Soybean using bar as a Selective Agent

On the basis of optimized soybean Agrobacterium-tumefaciens mediated transformation system,the trypsin inhibitor gene(sporamin)and chitinase gene(chitinase KDEL)were transformed into soybean cultivar YC-2 using germinated 1-2 days mature cotyledon node as explants and bar gene as a selectable agent in this paper.One hundred and fifteen putative transgenic soybean plants had been got and only 45 plants were positive transgenic plants identified by leaf painting 135 mg·L-1 Basta and polymerase chain reaction(PCR)methods.Six T0 positive plants were chosen,and four independent transgenic plants could be inherited into T1 progeny and the segregation rate of two T1 progenies were 3∶ 1,the other two T1 progenies were more than 3∶ 1.The results suggest that leaf painting 135 mg·L-1 Basta could be an effective and costless method to detect genetically modified soybeans progeny with bar gene as a selective agent.