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Effects of Retinoic Acid on Gastric Cancer Cells' Differentiation and Relevant Enzmye Activity

Yu Chen

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Abstract

Objective To explore effects of retinoic acid on gastric cancer cells' differentiation and relevant enzmye activity.Methods Treated with 10 -6 mol/L all trans retinoic acid(ATRA),gastric cancer cells,BGC 823 and MKN 45,were measured by flow cytometry for cell cycle distribution,by microplate reader for enzyme activity,by 3H TdR incorporation method for DNA synthetic speed and by MTT method for cell growth rate.Results After treated with ATRA,BGC 823 was lead to stay in G0/G1 phase,while LDH,ALP and β G activity was suppressed,the inhibition rates were 22 40%,32 94% and 41 35%,respectively.DNA synthetic speed was decreased by 41 43% and growth inhibition rate was 61 03%.Nevertheless,MKN 45 can't be changed as BGC 823.Conclusion BGC 823,but not MKN 45,can be induced differentiation and cell growth can be suppressed by ATRA.

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Objective To explore effects of retinoic acid on gastric cancer cells' differentiation and relevant enzmye activity.Methods Treated with 10 -6 mol/L all trans retinoic acid(ATRA),gastric cancer cells,BGC 823 and MKN 45,were measured by flow cytometry for cell cycle distribution,by microplate reader for enzyme activity,by 3H TdR incorporation method for DNA synthetic speed and by MTT method for cell growth rate.Results After treated with ATRA,BGC 823 was lead to stay in G0/G1 phase,while LDH,ALP and β G activity was suppressed,the inhibition rates were 22 40%,32 94% and 41 35%,respectively.DNA synthetic speed was decreased by 41 43% and growth inhibition rate was 61 03%.Nevertheless,MKN 45 can't be changed as BGC 823.Conclusion BGC 823,but not MKN 45,can be induced differentiation and cell growth can be suppressed by ATRA.

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Available abstract

Objective To explore effects of retinoic acid on gastric cancer cells' differentiation and relevant enzmye activity.Methods Treated with 10 -6 mol/L all trans retinoic acid(ATRA),gastric cancer cells,BGC 823 and MKN 45,were measured by flow cytometry for cell cycle distribution,by microplate reader for enzyme activity,by 3H TdR incorporation method for DNA synthetic speed and by MTT method for cell growth rate.Results After treated with ATRA,BGC 823 was lead to stay in G0/G1 phase,while LDH,ALP and β G activity was suppressed,the inhibition rates were 22 40%,32 94% and 41 35%,respectively.DNA synthetic speed was decreased by 41 43% and growth inhibition rate was 61 03%.Nevertheless,MKN 45 can't be changed as BGC 823.Conclusion BGC 823,but not MKN 45,can be induced differentiation and cell growth can be suppressed by ATRA.

Key concepts: Retinoic acid, Flow cytometry, Cell cycle, Chemistry, Molecular biology, Cell growth, Cancer cell, Cancer

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