2012•National Medical Frontiers of ChinaRequires access
The protective effect of simvastatin on kidney and its impact on expressions of TLR4 and MCP-1 in kidney tissues of type 1 diabetic rats
Liu Su-ju
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Abstract
Objective To explore the protective effect of simvastatin on kidney and its impact on expressions of TLR4 and MCP-1 in kidney tissues of type 1 diabetic rats.Methods Fifty-four Wistar rats were divided into three groups: normal control,diabetes and simvastatin treated groups.The blood glucose,serum insulin and 24h urinary protein were respectively determined in these groups at 4,8 and 12 weeks.In addition,the mRNA expressions and proteins of TLR4 and MCP-1 in the kidney tissues were respectively detected by using real time PCR(RT-PCR) and immunohistochemistry.The changes in ultrastructure of kidney tissues were observed under electron microscope in week 4,8,12.Results ①Compared with the normal control group,the diabetic group had a significant increase in the blood glucose,24h urinary protein excretion(P0.05),the mRNA expressions and proteins of TLR4 and MCP-1 in kidney tissues(P0.05),but serum insulin had a decrease(P0.05).The glomerular basement membrane was remarkably thickened.The mesangium and mesangial cells showed significant proliferation under electron microscope.②The simvastatin treated group had a obviously decrease in all the data mentioned above(P0.05);but the values tended to be higher as compared with the normal control group(P0.05).The pathological findings were also significantly improved,although something different from normal level.Conclusion Simvastatin may protect against kidney injury in diabetes through downregulating the expressions of TLR4 and MCP-1,hence inhibition of inflammatory reaction and reduing the kidney damage.