The Role of MCP-1 and ICAM-1 in Kidney Damaged Diabetic Nephropathy Rats and the Intervention Effect of Irbesartan

Objective:This experiment studied the role of monocyte chemoattractant protein-1( MCP-1) and intercellular adhesion molecule-1( ICAM-1) in Kidney damaged diabetic nephropathy rats and the effect of irbesartan on them.Methods:Using the high-sugar and high-fat diets combined with intraperitoneal injection of Streptozotocin to establish diabetic nephropathy rats model.Rats were randomly divided into normal control NC group,diabetic nephropathy DN group,irbesartan DI group,Testing 24 h urine volume,24 h urine protein quantitative( 24 h UTP),blood glucose( BG),blood muscle anhydride( Scr),urea nitrogen( BUN),and index of kidney( renal/weight,KI);Using HE stain to observe pathological morphology of each group,immunohistochemistry to observe the protein expression of MCP-1 and ICAM-1,RT-PCR to observe their mRNA expression.Results:Compared with the NC group,kidney pathological changes of DN group aggravated,24 h urine volume,24 h UTP,BG,Scr,BUN,KI and expressions of MCP-1 and ICAM-1 mRNA and protein in the renal tissues were significantly increased,with statistically significant differences( P 0.01);Compared with the DN group,BG,Scr and BUN of DI group relatively improved,with no statistically significant differences.Kidney pathological changes alleviated.The rest indices evidently reduced,with statistically significant differences( P 0.05).Conclusion:MCP-1and ICAM-1 may play an important role in the process of kidney damage in diabetic nephropathy;Irbesartan can reduce expressions of MCP-1and ICAM-1 in diabetic renal tissues,to some extent relieving the pathological damage of the kidney.