Study on rhG-CSF combined with improved CVP mobilization regimen in autologous peripheral blood stem cell transplantation in 41 patients with hematological malignancies and solid neoplasms

Objective:To evaluate the mobilization and hematopoietic reconstitution effects of improved CVP mobilization regimen plus rhG-CSF in 41 autologous peripheral blood stem cells transplantation(APBSCT)patients with hematological malignancies and solid neoplasms.Methods:Forty-one patients aged 39.6 years(range,18~67 years)(32 with hematological malignancies and 9 with solid neoplasms)were included from March 2001 to March 2006.The mobilization regimen was improved CVP regimen plus rhG-CSF.Mononuclear cells(MNC)harvest was performed when WBC recovered more than 4.0×109/L,and the MNC and CD34+ cells were counted.MNC was reinfused 48~72 hours after preconditioning of high dose chemotherapy.Results:WBC and PLT decreased to 1.0×109/L(0.1×109/L~0.8×109/L)and 40×109/L(4×109/L~36×109/L)respectively after mobilizing chemotherapy in all patients.Thirty four patients completed collection with a single apheresis,and 7 patients(5 patients with double APBSCT)underwent twice.The number of MNC and CD34+ cells obtained were 0.9×108/kg~8.3×108/kg(2.8±2.0×108/kg)and 1.1×106/kg ~9.4×106/kg(3.2±2.6×106/kg).No severe toxic reactions were observed.After conditioning,WBC recovered to 1.0×109/L at a median time of +11.3d(range +8~+16d),and PLT recovered to 50×109/L at a median time of +16.8d(range +13~+22d)except 3 patients.With a follow-up of 5 to 65 months after transplant,19 patients achieved complete remission(46.3%),13 patients partial remission(31.7%)and 9 patients no effects(22.0%).Conclusion:Improved CVP combined with rhG-CSF was a safe and effective regimen for the mobilization,which can be used in APBSCT with satisfying outcomes.