Studies on the Culture Conditions for Neutral Protease Solid Fermentation by Aspergillus oryzae

using Aspergillus oryzae Y10 as the test strain,medium for neutral protease solid fermentation was studied and optimized.The suitable composition (% ) of the medium is as follows: wheat bran 9, yeast powder 1,lactose 1, water 9 , pH 7. 0. The optimal cultural temperature is 30℃, culture duration 72 hours, and inoculating 1.8 ×108 spores each gram medium. Under this optimal conditions, the neutral protease activity is as high as 4286.1 U/g.