Study on plasma TNF level and TNFα-850C/T polymorphisnis in patients with allergic rhinitis

Objective To investigate the relationship between plasma levels of tumor necrosis factor(TNF) or its polymorphism and allergic rhinitis.Methods Plasma level of TNF was measured by RIA,the polymorphism of TNFα-850C/T gene was screened by PCR-RELP.Results The gene frequency of TNFα-850C/T in allergic rhinitis group was as followed: TNFα-850 C/C,T/C,T/T genotype frequency: 0.7445,0.2333,0.0222;TNFα-850 C,TNFα-850 T Gene frequency: 0.8611,0.1389.TNFα850 C/C,T/C,T/T genotype frequency in control group was as followed : 0.7850,0.1963,0.0187,TNFα-850 C,TNFα-850 T Gene frequency: 0.8832,0.1168,there were no significant differences in the frequencies of TNFα-850C/T gene between allergic rhinitis patients and control group(P 0.05).Allergic rhinitis group showed significantly higher plasma level of TNF than control group(P0.05).Conclusion Plasma TNF increased in allergic rhinitis.The results suggested that TNF playn important role in the process of allergic rhinitis and no evident association was found between TNFα-850C/T polymorphism and allergic rhinitis