RP-HPLC Determination of Three Medicinal Bioactive Components in Different Parts of Swertia mussotii Franch

Objective:To establish an HPLC method for determining the contents of three medicinal bioactive components:gentiopicroside(S_1),mangiferin(S_2)and 7-O-β-D-xylopyranosyl-1,8-dihydroxy-3- methoxyxanthone(S_3)in different parts of wild and cultivated Swertia mussotii Franch were determined by RP- HPLC.Methods:The analysis was performed on a Kromasil C_(18)(250 mm×4.6 mm,5μm).The mobile phase con- sisted of CH_3OH and 0.02% aqueous phosphoric acid.The gradient condition was O min→50 min→55 min, CH_3OH 20%→80%→100%.The flow rate was 1 mL·min~(-1).The detection wavelength was at 260 nm.Results: The good linear relationships and recoveries were obtained under the optimum conditions for every component.Con- clusion:The method is simple,rapid and reliable.The flower of this herb contains more three medicinal bioactive components than its other parts do.