Determination of Three Medicinal Bioactive Components of Swertia franchetiana H.Smith

[Objective] The purpose was to determine three medicinal bioactive components in different parts of Swertia franchetiana H.Smith.[Method]The content of swertiamarin,gentiopicroside and mangiferin in Swertia franchetiana H.Smith were determined by reversed-phase high performance liquid chromatography.[Result]The optimum conditions of determining three medicinal bioactive components in different parts of Swertia franchetiana H.Smith were as follow: a Kromasil C18(250 mm×4.60 mm,5 μm);the mobile phase was composed of CH3OH and 0.02% phosphoric acid in water;the gradient condition was 0-25 min,CH3OH 20%-50%;the flow rate was 1 ml/min;the detection wavelength was 260 nm.The good linear relationships and recoveries were obtained under the optimum conditions.[Conclusion]The content of gentiopicroside is most high in the root,swertiamarin and mangiferin have higher content in the floral part of Swertia franchetiana H.Smith.