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2005•The Chinese Journal of DermatovenereologyRequires access

Cloning of Human anti-TRP1 Antibodies From a Phage Antibody Library

Gang Wang

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Abstract

Objective To clone human phage antibodies against TRP-1( tyrosinase related protein 1) from a phage antibody library.Methods Panning of the phage antibody library against TRP-1 antigen was conducted to select specific single-chain antibodies. The antigen binding activity and the specificity of the isolated binders were determined with ELISA.Results After 4 rounds of adhension-amplification-elution panning, 8 clones of scFv which could bind specifically to TRP-1 were obtained. The genes of them then were sequenced and analyzed.Conclusion It is feasible to isolate human anti-TRP-1 phage antibodies by-passing immunization using phage antibody library.

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What this paper is about

Objective To clone human phage antibodies against TRP-1( tyrosinase related protein 1) from a phage antibody library.Methods Panning of the phage antibody library against TRP-1 antigen was conducted to select specific single-chain antibodies. The antigen binding activity and the specificity of the isolated binders were determined with ELISA.Results After 4 rounds of adhension-amplification-elution panning, 8 clones of scFv which could bind specifically to TRP-1 were obtained. The genes of them then were sequenced and analyzed.Conclusion It is feasible to isolate human anti-TRP-1 phage antibodies by-passing immunization using phage antibody library.

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Available abstract

Objective To clone human phage antibodies against TRP-1( tyrosinase related protein 1) from a phage antibody library.Methods Panning of the phage antibody library against TRP-1 antigen was conducted to select specific single-chain antibodies. The antigen binding activity and the specificity of the isolated binders were determined with ELISA.Results After 4 rounds of adhension-amplification-elution panning, 8 clones of scFv which could bind specifically to TRP-1 were obtained. The genes of them then were sequenced and analyzed.Conclusion It is feasible to isolate human anti-TRP-1 phage antibodies by-passing immunization using phage antibody library.

Key concepts: Panning (audio), Phage display, Antibody, clone (Java method), Antigen, Peptide library, Phagemid, Molecular biology

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