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SRAP Analysis on Genetic Diversity of Chinese Kale Germplasm

Guiquan Zhang

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Abstract

The morphological characteristics of 56 Chinese kale accessions were investigated and the DNA polymorphism was analyzed by sequence related amplified polymorphism(SRAP)markers.A total of 747 bands were amplified using 24 primers selected from 312 primers,of which147 were polymorphic,The polymorphic percentage was 19.6%.Among these germplasm the genetic similarity was ranged from 0.524 to 0.884 by software NTSYSpc2.1 based on SRAP results,Cluster analysis showed that 56 Chinese kale germplasm were divided into six groups on genetic similarity of 0.66.Because Chinese kale was from south China,the genetic diversity of Chinese kale was smaller than other Brassica vegetables.

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What this paper is about

The morphological characteristics of 56 Chinese kale accessions were investigated and the DNA polymorphism was analyzed by sequence related amplified polymorphism(SRAP)markers.A total of 747 bands were amplified using 24 primers selected from 312 primers,of which147 were polymorphic,The polymorphic percentage was 19.6%.Among these germplasm the genetic similarity was ranged from 0.524 to 0.884 by software NTSYSpc2.1 based on SRAP results,Cluster analysis showed that 56 Chinese kale germplasm were divided into six groups on genetic similarity of 0.66.Because Chinese kale was from south China,the genetic diversity of Chinese kale was smaller than other Brassica vegetables.

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Available abstract

The morphological characteristics of 56 Chinese kale accessions were investigated and the DNA polymorphism was analyzed by sequence related amplified polymorphism(SRAP)markers.A total of 747 bands were amplified using 24 primers selected from 312 primers,of which147 were polymorphic,The polymorphic percentage was 19.6%.Among these germplasm the genetic similarity was ranged from 0.524 to 0.884 by software NTSYSpc2.1 based on SRAP results,Cluster analysis showed that 56 Chinese kale germplasm were divided into six groups on genetic similarity of 0.66.Because Chinese kale was from south China,the genetic diversity of Chinese kale was smaller than other Brassica vegetables.

Key concepts: Germplasm, Genetic diversity, Biology, Genetic similarity, Brassica, Polymorphism (computer science), Genetic relationship, Horticulture

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