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Reproduction and Genotype Identification of Complement 3 Gene Knockout Mice

Qiang Zou

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Abstract

Objective This study was to explore the method to breed and identify complement 3 gene knockout mice.Methods The heterozygote of complement 3 gene knockout mice(C3 +/-) were bred and reproduced.Wild genotype(C3 +/ +),heterozygote genotype(C3 +/-),and homozygote genotype(C3-/-) would appear in offspring.The genotypes were characterized using ELISA and PCR.Result The heterozygote mice bred 135 offspring.38 mice are wild type,68 mice are heterozygote,and 29 mice are homozygote.By χ2text,the segregate ratio of offspring was fitted to the Mendelian ratio.Conclusion Appropriate methods for breeding,reproducing,and identifying are the effective way for acquiring complement 3 gene knockout mice from heterozygote.

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What this paper is about

Objective This study was to explore the method to breed and identify complement 3 gene knockout mice.Methods The heterozygote of complement 3 gene knockout mice(C3 +/-) were bred and reproduced.Wild genotype(C3 +/ +),heterozygote genotype(C3 +/-),and homozygote genotype(C3-/-) would appear in offspring.The genotypes were characterized using ELISA and PCR.Result The heterozygote mice bred 135 offspring.38 mice are wild type,68 mice are heterozygote,and 29 mice are homozygote.By χ2text,the segregate ratio of offspring was fitted to the Mendelian ratio.Conclusion Appropriate methods for breeding,reproducing,and identifying are the effective way for acquiring complement 3 gene knockout mice from heterozygote.

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Available abstract

Objective This study was to explore the method to breed and identify complement 3 gene knockout mice.Methods The heterozygote of complement 3 gene knockout mice(C3 +/-) were bred and reproduced.Wild genotype(C3 +/ +),heterozygote genotype(C3 +/-),and homozygote genotype(C3-/-) would appear in offspring.The genotypes were characterized using ELISA and PCR.Result The heterozygote mice bred 135 offspring.38 mice are wild type,68 mice are heterozygote,and 29 mice are homozygote.By χ2text,the segregate ratio of offspring was fitted to the Mendelian ratio.Conclusion Appropriate methods for breeding,reproducing,and identifying are the effective way for acquiring complement 3 gene knockout mice from heterozygote.

Key concepts: Heterozygote advantage, Genotype, Offspring, Biology, Gene knockout, Genetics, Knockout mouse, Mendelian inheritance

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