Breeding,reproducing and identification of SRC-1 knock-out mice

Objective To breed and identify SRC-1 knock-out mice.Methods Wild male mice and female SRC-1 knock-out mice were bred and reproduced.Heterozygote genotype mice appeared in offspring after mating of wild mice and homozygote mice.Then to let heterozygote mice for mating with each other.So the wild type,heterogote type and homozygote type mice were acquired according to Mendel's law.Genome DNA extracted from tails of mice was subjected to PCR for genotype identification.Male and female homozygote mice once acquired were choiced to mate with each other for more homozygote baby.Results Breeding and reproducing were succeeded and more homozygote genotype mice were produced.Conclusion Proper methods of breeding,reproducing and identification will result in more homozygote mice.