Selection of microorganisms of degraded anthracene and analysis of its middle products

A predominant PAHs-degraded strain was obtained by the selective enrichment culture from ocean sediment.The microorganisms with ability to degrade anthracene were obtained with anthracene as the solo carbon source for growth.The bacteria were preliminary identified as Flavobacterium based on its morphological and physio-biochemcial characteristics.In a mineral salts medium with initial anthracene concentration of 50mg/L,the microbial population and the strain could degrade anthracene well,and the removal rate of anthracene in the medium was 77.6% in 5 days.The study on selectivity enzyme-degraded of PAHs was found that the degradation efficiency to anthracene was better than that to phenanthrene in a mixture culture with 15mg/L of naphthalene,anthracene and phenanthrene.1,2-Benzenedicarboxylic acid-bis(2-methylpropyl) ester,9,10-anthracenedione and dibutylphthalate were identified as the major metabolites in the culture by GC/MS analysis.Additional,the phthalic acid pathway to anthracene was detected as a metabolizing process.